Journal: Infectious Diseases and Therapy

Article Title: Multicentre Performance Evaluation of the Elecsys Anti-SARS-CoV-2 Immunoassay as an Aid in Determining Previous Exposure to SARS-CoV-2

doi: 10.1007/s40121-021-00504-9

Figure Lengend Snippet: COI distribution in patient samples presumed negative for SARS-CoV-2 antibodies ( n = 9575). COI cut-off index, SARS-CoV-2 severe acute respiratory syndrome coronavirus 2

Article Snippet: The Elecsys Anti-SARS-CoV-2 immunoassay demonstrated significantly higher specificity versus LIAISON SARS-CoV-2 S1/S2 IgG (99.71% vs. 98.48%), EUROIMMUN Anti-SARS-CoV-2 IgG (100.00% vs. 94.87%), ADVIA Centaur SARS-CoV-2 Total (100.00% vs. 87.32%) and iFlash SARS-CoV-2 IgM (100.00% vs. 99.58%) assays, and comparable specificity to ARCHITECT SARS-CoV-2 IgG (99.75% vs. 99.65%) and iFlash SARS-CoV-2 IgG (100.00% vs. 100.00%) assays.

Techniques:

Journal: Infectious Diseases and Therapy

Article Title: Multicentre Performance Evaluation of the Elecsys Anti-SARS-CoV-2 Immunoassay as an Aid in Determining Previous Exposure to SARS-CoV-2

doi: 10.1007/s40121-021-00504-9

Figure Lengend Snippet:

Article Snippet: The Elecsys Anti-SARS-CoV-2 immunoassay demonstrated significantly higher specificity versus LIAISON SARS-CoV-2 S1/S2 IgG (99.71% vs. 98.48%), EUROIMMUN Anti-SARS-CoV-2 IgG (100.00% vs. 94.87%), ADVIA Centaur SARS-CoV-2 Total (100.00% vs. 87.32%) and iFlash SARS-CoV-2 IgM (100.00% vs. 99.58%) assays, and comparable specificity to ARCHITECT SARS-CoV-2 IgG (99.75% vs. 99.65%) and iFlash SARS-CoV-2 IgG (100.00% vs. 100.00%) assays.

Techniques: Recombinant, Diagnostic Assay, Infection

Journal: Frontiers in Pharmacology

Article Title: Bictegravir Plus Tenofovir Alafenamide Nanoformulation as a Long-Acting Pre-Exposure Prophylaxis Regimen: Application of Modeling to Design Non-Human Primate Pharmacokinetic Experiments

doi: 10.3389/fphar.2020.603242

Figure Lengend Snippet: Comparison of BIC and TAF/TFV NCA parameters between MBPK-predicted nBIC-TAF and BIKTARVY in Non-human Primates.

Article Snippet: From nBIC-TAF , BIC’s C max was lower than BIKTARVY’s C max (3.7 vs. 19 μg/ml); The end concentrations C end (i.e., concentrations at T end ) were lower for BIC than BIKTARVY (0.94 vs. 2.3 μg/ml); Note, however, that we report C end for BIC from nBIC-TAF at 28 days, whereas BIKTARVY’s C end is at 1 day.

Techniques:

Journal: Scientific Reports

Article Title: Differential type I interferon response and primary airway neutrophil extracellular trap release in children with acute respiratory distress syndrome

doi: 10.1038/s41598-020-76122-1

Figure Lengend Snippet: Demographic and clinical characteristics of study participants.

Article Snippet: The proportion of children with PARDS had fewer ventilator-free days over 20 days compared with children without PARDS (33/35, 94.3% vs. 22/42, 52.4%, Chi-squared likelihood ratio test = 18.67, p value < 0.0001).

Techniques: Virus

Journal: Scientific Reports

Article Title: Differential type I interferon response and primary airway neutrophil extracellular trap release in children with acute respiratory distress syndrome

doi: 10.1038/s41598-020-76122-1

Figure Lengend Snippet: Neutrophil activation and degranulation markers in the tracheal aspirate samples from children with acute respiratory failure with and without PARDS. The gating strategy for single, living, CD66b + neutrophils is shown ( A–C ). The total number of neutrophils in the sample ( D ) was determined by multiplying the total number of cells in the tracheal aspirate sample by the percentage of living CD66b + cells (neutrophils) in the sample ( E ). The mean fluorescence intensity (MFI) of ( F ) CD63 ( n = 17, No PARDS, n = 10, Yes PARDS), ( G ) sphingosine 1-phosphate receptor 3 (S1PR3) ( n = 17, No PARDS, n = 10, Yes PARDS), ( H ) CD16 ( n = 17, No PARDS, n = 10, Yes PARDS), and ( I ) Arginase 1 (Arg1, n = 16, No PARDS, n = 10, Yes PARDS) on the surface of airway neutrophils of children with PARDS (gray boxplots) compared with children without PARDS (white boxplots) are shown. Each circle (No PARDS) and each square (Yes PARDS) represent an individual patient. A cytospin and Diff-Quik stained processed airway sample is shown ( J ). Scale bars indicate 50 microns. Two-tailed Mann–Whitney U test. ** p < 0.01, * p < 0.05.

Article Snippet: The proportion of children with PARDS had fewer ventilator-free days over 20 days compared with children without PARDS (33/35, 94.3% vs. 22/42, 52.4%, Chi-squared likelihood ratio test = 18.67, p value < 0.0001).

Techniques: Activation Assay, Fluorescence, Diff-Quik, Staining, Two Tailed Test, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Differential type I interferon response and primary airway neutrophil extracellular trap release in children with acute respiratory distress syndrome

doi: 10.1038/s41598-020-76122-1

Figure Lengend Snippet: Phosphorylated STAT-1 (P-STAT1 (Y701)) and STAT1 expression in primary airway cells in children with and without PARDS. Cells were gated on forward and side-scatter. Histograms of primary flow data along with cell counts are noted. Histogram data is scaled using the modal function in FlowJo analysis software ( A ). Boxplots of the mean fluorescence intensity (MFI) of P-STAT1 ( B ) and STAT-1 ( C ) along with the percent cells positive for P-STAT1 ( D ) or STAT1 ( E ) are shown. The fluorescence minus 1 (FM1) histogram was used to draw the percent positive gate for P-STAT1 and STAT-1, respectively. FM1 (gray histogram), No PARDS (circles/blue boxplot and blue histograms) and PARDS (squares/red boxplot) and red histograms). For all analyses, n = 19 (No PARDS), n = 15 (Yes PARDS). Two-tailed Mann–Whitney U test. * p < 0.05.

Article Snippet: The proportion of children with PARDS had fewer ventilator-free days over 20 days compared with children without PARDS (33/35, 94.3% vs. 22/42, 52.4%, Chi-squared likelihood ratio test = 18.67, p value < 0.0001).

Techniques: Expressing, Software, Fluorescence, Two Tailed Test, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Differential type I interferon response and primary airway neutrophil extracellular trap release in children with acute respiratory distress syndrome

doi: 10.1038/s41598-020-76122-1

Figure Lengend Snippet: Anti-viral and interferon (IFN) stimulated gene (ISG) expression in control children and in children with and without PARDS. ( A ) IFIT1 (IFN Induced proteins with Tetraicopaptide repeats), n = 10 with 3 outliers removed (No PARDS), n = 7 (Yes PARDS), ( B ) ISG15, n = 12 with 1 outlier removed (No PARDS), n = 7 (Yes PARDS), ( C ) MX1, n = 13 with no outliers (No PARDS), n = 7 (Yes PARDS). Two-tailed Mann–Whitney U test. The p values are noted above the boxplots.

Article Snippet: The proportion of children with PARDS had fewer ventilator-free days over 20 days compared with children without PARDS (33/35, 94.3% vs. 22/42, 52.4%, Chi-squared likelihood ratio test = 18.67, p value < 0.0001).

Techniques: Expressing, Control, Two Tailed Test, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Differential type I interferon response and primary airway neutrophil extracellular trap release in children with acute respiratory distress syndrome

doi: 10.1038/s41598-020-76122-1

Figure Lengend Snippet: Airway neutrophil extracellular trap (NET) release by PARDS status and ventilator-free days. NET release as measured by MPO-DNA enzyme-linked immunosorbent assay ( A ) from cell-free tracheal aspirate samples of children without PARDS (blue boxplot, n = 33, where each circle is an individual patient) versus with PARDS (red boxplot, n = 42, where each square is an individual patient). ( B ) MPO-DNA complexes in tracheal aspirates were stratified by those children who did not have more than 20 ventilator-free days (VFD) (white boxplot, n = 22, where each circle is an individual patient) and those children who did have more than 20 VFD (gray boxplot, n = 53, where each square is an individual patient). Boxplots depict median (line), 25th–75th interquartile range (box edges), and min to max values (whiskers). Values are normalized to a standardized value of 1 and analyzed using a two-tailed Mann–Whitney U test, *p < 0.05, ** p < 0.01.

Article Snippet: The proportion of children with PARDS had fewer ventilator-free days over 20 days compared with children without PARDS (33/35, 94.3% vs. 22/42, 52.4%, Chi-squared likelihood ratio test = 18.67, p value < 0.0001).

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Differential type I interferon response and primary airway neutrophil extracellular trap release in children with acute respiratory distress syndrome

doi: 10.1038/s41598-020-76122-1

Figure Lengend Snippet: Airway neutrophils are activated in intubated children with compared to children without PARDS. Surface expression of the primary granule exocytosis marker, CD63, and the lipid signaling G protein-coupled receptor, sphingosine-1-phosphate receptor 3 (S1PR3), are higher in children with versus without PARDS. The type I interferon (IFN) signaling pathway transcription factor, STAT1, is upregulated and phosphorylated, and transcript levels of ISG15 is increased in children with versus without PARDS. Neutrophil extracellular trap (NET) release is regulated by a NADPH oxidase (NOX) respiratory burst (reactive oxygen species (ROS) triggered mechanism and an intracellular calcium-dependent trigger. It is not known which trigger dominates in the airways of children with PARDS. Children with PARDS have elevated levels of NETs in their airways as detected by myeloperoxidase (MPO)-DNA complexes in our study. Elevated NET levels are associated with a higher number of ventilator-free days (VFD) over 20 days in a 28-day period (i.e. if the child survived, then they were more likely to spend ≥ 7 days endotracheally intubated and mechanically ventilated). Created with BioRender.com using the web version, which may be accessed at https://biorender.com/ , with a paid individual subscription granting permission to publish in journals.

Article Snippet: The proportion of children with PARDS had fewer ventilator-free days over 20 days compared with children without PARDS (33/35, 94.3% vs. 22/42, 52.4%, Chi-squared likelihood ratio test = 18.67, p value < 0.0001).

Techniques: Expressing, Marker